Cyclospora Resources
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- Current Knowledge and Future Directions for Cyclospora cayetanensis Research and Its Surrogates
Life Cycle and Transmission of Cyclospora cayetanensis: Knowns and Unknowns
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CPS Funded Research
The 2027 CPS Research Priorities include specific research questions focused on Cyclospora. In addition, CPS currently has a research project underway to better understand environmental occurrence, transmission and persistence of Cyclospora pathways that may precede produce contamination. This work may help identify best management practices opportunities for growers and water managers to earlier intervention and reduce potential risks mitigation.
Detection & Laboratory Methods
Focusing on improving recovery, detection, and identification of Cyclospora.
- An immunomagnetic separation method for concentrating and increasing the recovery efficiency of Cyclospora
- Validation of novel DNA isolation procedures from limited numbers of Cyclospora oocysts
- Development of an infrared-functionalized microbalance sensor for Cyclospora cayetanensis detection and differentiation
- Methods for the detection of diverse parasites on packaged salads based on (viable) oocysts
Viability Assessment
Research developing methods to determine whether Cyclospora oocysts remain alive and capable of causing infection.
Agricultural Water Monitoring & Surveillance
Research improving our understanding of where Cyclospora occurs and how it moves through agricultural water systems.
- Cyclospora cayetanensis monitoring in agricultural water
- Sources and prevalence of Cyclospora cayetanensis in Southeastern US water sources and growing environments
- The prevalence of Cyclospora in water and produce
- Cyclospora prevalence in irrigation water in fresh produce growing regions
- Cyclospora: potential reservoirs and occurrence in irrigation waters
- Microbial characterization of irrigation waters using rapid, inexpensive and portable next generation sequencing technologies
- Improved sampling and analytical methods for testing agricultural water for pathogens, surrogates and source tracking indicators
Water Treatment & Filtration
Research evaluating technologies to reduce Cyclospora contamination in agricultural water.
- AFECCT: Assessing filtration efficacy for Cyclospora control
- Analysis of the presence of Cyclospora in waters of the Mid-Atlantic States and evaluation of removal and inactivation by filtration
- Determination of physical and chemical mechanisms to prevent Cyclospora infection
Related Scientific Publications
Biology & Life Cycle
Research focused on understanding the parasite's biology, transmission, and survival.
Detection & Viability Methods
Research developing new methods to determine whether parasite oocysts remain viable and infectious.
- Validating the updated BAM chapter 19b method for the detection of Cyclospora cayetanensis in outbreak-linked fresh produce
- Deep learning-based detection and viability assessment of Eimeria oocysts
- Morphological and autofluorescence assessment of oocysts differentiate live from dead coccidian parasites
Mitigation Strategies
Research evaluating interventions to reduce parasite survival and infectivity.
- UV radiation at 222, 254, and 282 nm inhibits sporulation and suppresses infectivity of Eimeria acervulina oocysts
- Aqueous Ozone Exposure Inhibits Sporulation in the Cyclospora cayetanensis Surrogate Eimeria acervulina
Water Filtration
Research evaluating filtration technologies to reduce parasite contamination in agricultural water.
- Filters comprised of sand and Zero Valent Iron hold promise as tools to mitigate risk posed by Cyclospora cayetanensis oocysts
- Zero-Valent Iron and Sand Filtration Reduces Levels of Cyclospora cayetanensis Surrogates, Eimeria tenella and Eimeria acervulina, in Water
Epidemiology & Surveillance
Research examining the occurrence, distribution, and prediction of Cyclospora in agricultural environments.