Summary of Awards to Date

Integrating research, teaching, and outreach efforts to faciliate industry application of molecular subtyping for foodborne pathogens.

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Sep. 1, 2008 - Aug. 31, 2011

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Funding Agency


Amount Awarded



Kendra Nightingale, Ph.D.
Colorado State University


Wiedmann, M.


Most recent estimates project that each year in the U.S. foodborne pathogens caue in 76 million illnesses, 325,000 hospitalizations, and 5,000 deaths. Furthermore, 1,100 of 1,800 deaths due to known foodborne pathogens can be attributed to a few key pathogens, including shiga toxin producing Escherichia coli, Listeria monocytogenes, and Salmonella. "DNA fingerprinting" or molecular subtyping techniques can differentiate isolates belonging to a given foodborne pathogen beyond the species level and molecular subtyping is routinely in outbreak investigations to identify a food source responsible cases of foodborne illness. While regulatory agencies routinely subtype foodborne pathogen isolates from clinical cases of human foodborne illness, these molecular subtyping data are not publicly accessible and subtype data for isolates obtained from environmental sources are critical to determine the significance of a shared human clinical and food subtype. The overall goal of this project is to expand research, teaching, and outreach efforts to facilitate the wide-spread implementation of molecular subtyping to track and control foodborne pathogens. In order to accomplish this goal, we will complete the following objectives: Objective 1. Generate molecular subtyping data and populate the publicly available PathogenTracker database with subtype data for key foodborne pathogens isolated from non-food associated environmental sources. Objective 2. Develop sustainable systems for adding human clinical, animal clinical, and food isolate molecular subtyping data for key foodborne pathogens to PathogenTracker. Objective 3. Develop and conduct workshops on molecular subtyping for food industry professionals. Objective 4. Develop and conduct teaching modules on molecular subtyping for undergraduate and graduate students.