Summary of Awards to Date

Attenuated strains of Salmonella for use as live vaccine.

Read Full Publication...

Date

Feb. 1, 2009 - Sep. 30, 2011

Award Number

WIS01380

Funding Agency

Center for Produce Safety

Investigator

Amin Fadl, Ph.D.
University of Wisconsin-Madison

Summary

Salmonella causes large numbers of human infections due to consumption of contaminated food. The disease is characterized by abdominal cramps, diarrhea, nausea, vomiting, and high number of bacteria in the blood. The Center for Disease Control and prevention estimates that 1.5 million people per year are affected by Salmonella-related illnesses. Long term goals of this research are to develop strategies to protect animal from Salmonella infection and hence reduce human outbreaks. We created weakened (attenuated) type of Salmonella for use as vaccine to protect animals against Salmonella infection. The attenuation of Salmonella was made by deletion of. gidA gene (code for bacterial factors (virulence) enable them to cause diseases. Therefore, this deletion makes Salmonella less capable of causing infections in animals and humans. The purpose of this study is to test the ability of attenuated Salmonella to invade cultured intestinal cell and to induce the release inflammatory mediators. We will test the ability of such attenuated Salmonella to cause death in mice infected with different bacterial doses, compared to mice infected with the wild-type (non-attenuated) Salmonella. It is essential to examine whether injection of mice with the attenuated bacteria would provide protection against lethal infection of Salmonella. This study will also tell us about how these animals are protected from death when infected with Salmonella. In this experiment, mice will also be infected with non-lethal dose of bacteria and blood samples and tissues will be collected and examined for the toxic effect of the bacteria. Such information is critical to determine potential use of attenuated bacteria as a vaccine candidate.